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Apligraf® product details.

See how to apply Apligraf to VLUs and DFUs. Also, view additional resources.

How to apply Apligraf

Diabetic foot ulcer application

Venous leg ulcer application

Application steps1,2

Preparing the wound bed

Apply Apligraf to a clean, noninfected, and debrided wound after irrigating the wound with a noncytotoxic solution. Ensure hemostasis following debridement.

Step 1: Preparing Apligraf


Before opening, check the package's expiration date and pH to ensure both are within range


Apligraf is packaged with the epidermal (matte) side facing up and the dermal (glossy) side facing down

  • The dermal side rests on a polycarbonate membrane; be sure to not inadvertently remove it with Apligraf

Open the pack and remove Apligraf using aseptic techniques

  • Gently remove by lifting from the edge
  • Apligraf must be used within 15 minutes of opening

Prior to application, Apligraf may be placed on a saline-soaked gauze and/or fenestrated to allow for drainage

Expiration date and pH ranges for Apligraf 1A
Apligraf with the epidermal (matte) side facing up 1B
Apligraf lifted from its container with forceps 1C
Apligraf placed on saline-soaked gauze for drainage 1D

Step 2: Applying Apligraf


Place Apligraf over the wound with the dermal (glossy) side directly in contact with the wound surface


Using a saline-moistened cotton tip applicator, smooth Apligraf onto the wound bed so there are no pockets or wrinkled edges

Apligraf applied directly in contact with the wound surface 2A-2B

Step 3: Applying dressing


Cover Apligraf using a non-adhesive primary dressing


Anchor Apligraf using clinician’s choice of fixation


Apply a secondary, non-occlusive dressing to create a bolster

Apligraf covered with a non-adhesive primary dressing 3A-3B

Compress/offload and follow-up

  • Apligraf should always be used with appropriate compression or offloading as well as good wound-care practices
  • Reassess the wound weekly, and reapply Apligraf weekly as long as the wound continues to respond*
  • Debride the wound while not disrupting healing tissue
  • Clean the wound with a noncytotoxic solution
Doctor wrapping a leg in compression after applying Apligraf
Foot offloaded after receiving an Apligraf treatment
Wound that reduced in size with the help of Apligraf
*The safety and effectiveness of Apligraf have not been established for patients receiving greater than 5 device applications. In the pivotal trials for VLUs and DFUs, patients received up to 5 applications (over 3 weeks for VLUs and over 4 weeks for DFUs). The average number of applications per VLU patient was 3.3 and the average number for DFU patients was 3.9.1-3

Downloadable resources

Apligraf factsheet

Product Description
Total Size
HCPCS CodeUPC Number
APLIGRAF-COMApligraf (living, bi-layered skin substitute)4444Q410118474-000000

Proven results in clinical trials and real-world settings

Examine the clinical and real-world evidence for Apligraf, or contact an Organogenesis Tissue Regeneration Specialist today to get more information about how Apligraf can help your VLU and DFU patients.

Contact us

Please refer to the Apligraf Package Insert for complete prescribing information.


  1. Apligraf [package insert]. Canton, MA: Organogenesis Inc; 2017.
  2. Data on file. Organogenesis Inc.
  3. Veves A, et al. Diabetes Care. 2001;24(2):290-295.